Biochemistry 900 (905): Advanced Topics in Biochemistry

Bio-Macromolecular NMR spectroscopy, 3 cl. Call # 22112-9

Spring Quarter 2009, MW 9:00-10:18 am, 668 BioSci

Instructors: M. Foster (foster.281), J. Wu (wu.473@osu.edu)

 

Description: This course, aimed at graduate students and advanced undergraduates, will emphasize the application of NMR spectroscopy to biochemical problems and structural biology. The fundamentals of NMR spectroscopy will be described qualitatively, relying on the vector model and product operators to describe evolution of magnetization; thus, no background in quantum mechanics or matrix algebra is required. Grades will be assigned based on homework exercises, one take-home exam and a special topics presentation.

 

Syllabus:

  1. The vector model
  2. CW and FT NMR
  3. Chemical shift and coupling
  4. Product operators
  5. Phase cycling
  6. Coherence selection
  7. Heteronuclear NMR (INEPT, magnetization transfer)
  8. Multidimensional NMR (2D, 3D, 4D)
  9. Triple resonance experiments
  10. Protein assignment strategies
  11. Nucleic acid NMR and assignments
  12. Strategies for large macromolecule NMR (TROSY, deuteration)
  13. Structure determination
  14. Relaxation/Dynamics/Exchange
  15. Ligand binding (shift perturbations, fast/slow exchange, heteronuclear filters, saturation transfer, protein-protein, protein-DNA, protein-small molecule)
  16. Shaped/composite pulses, decoupling
  17. Practical aspects
    1. Sample preparation
    2. Labeling
    3. Data processing and analysis
    4. NMRPipe, NMRView, Topspin, XWINNMR, CARA, SPARKY
    5. Cryoprobes
    6. Residual dipolar couplings

 

Text: Rule & Hitchens, Fundamentals of Protein NMR Spectroscopy, 2006, Springer [$85]

 

Other texts:

Cavanagh et al., Protein NMR Spectroscopy 2006, Elsevier/Academic Press [$80 from Amazon]

Teng, Structural Biology; Practical NMR Applications, (2005)  [$90, Springer]

Keeler, Understanding NMR, 2005, Wiley [$50]

Pochapsky & Pochapsky, NMR for Physical and Biological Scientists (2006) [$83, Garland]

Downing, Protein NMR Techniques, 2nd Ed., (Methods in Molecular Biology, v. 278, 2004) [$115, Humana Press]

Hore et al., NMR: The Toolkit  (2000) [$25, Oxford University Press]

Levitt , Spin Dynamics (2001) [$85, Wiley]