Documentation

• Instruction Manual (370Kb)
• Software Manual parts I (1.2Mb) and II (1.6Mb)
• Planning your CD experiment (Ross Wilson)
  • Time required, liquid N2, sample, buffer, etc. (82.5 Kb)
• CD usage protocol (Ross Wilson)
  • Routine use of instrument (71.4 Kb)
• Ordering liquid Nitrogen (Takes ~3 days)
  • Order through OSU Stores Gas Cylinder Warehouse
  • http://osustores.osu.edu/stores/gascylinder.aspx
• Issues with Avid CD and calibration (Dr. Tom Magliery, 2010/10/05)
  • Aviv CD spectrometer may have issues for specific wavelength regions
  • This may be assessed by calibration (119 kb)

Calendar (instrument availability)

• CD calendar
• Scheduling: Vineet Gaur (vineetgaur1982@gmail.com) / 880 Biosci. / 688-3706
• Note that the liquid nitrogen can take up to three days to be refilled, so allow appropriate time when planning your experiments.

Information

• 1.4 mL cell volume, 285 uL syringe volume
• Microcal VP-ITC user manual (5.3 Mb)
• Maintenance and benchmarking log (<100 kB)
• Microcal web site

Calendar (instrument availability)

• VP-ITC calendar
• Scheduling: Elihu Ihms (ihms.2@osu.edu) / 732 Riffe / 688-8146 (ask for Eli)

Information

• 280 uL cell volume, 60 uL syringe volume
• Microcal iTC200 user manual (922 Kb)
• Investigation of periodicity in iTC200 Injections (Elihu Ihms, pdf)
• Maintenance and benchmarking log (<100 kB)
• Microcal web site

Calendar (instrument availability)

• iTC200 calendar
• Scheduling: Elihu Ihms (ihms.2@osu.edu) / 732 Riffe / 688-8146 (ask for Eli)

Information

• This instrument is no longer available for use at OSU, please contact Elihu Ihms for comparison information.
• 950 uL cell volume, 100 or 250 uL syringe volume
• Nano ITC website

Articles

• ITC review (Jelesarov & Bosshard, 1999) (271.1Kb)
  • Excellent introductory review of calorimetry
• ITC review (Holdgate (Microcal), 2001) (1.0 Mb)
  • Another nice review for background, analysis, and interpretation
• Microcal ITC analysis using Orign software, 2004 (1.3 Mb)
  • For detailed analysis of ITC data using Origin program
• Microcal ITC troubleshooting guide (Microcal, date unknown) (324 Kb)
  • For commonly encountered problems

Experimental design and simulation

• Planning your ITC experiment (Ian Kleckner)
  • Essential reading material for any ITC user (62 kB)
• Sample preparation (Vanderbilt) (link)
  • Practical advice from the Microcal manual
• ITC sample requirement calculator (Ian Kleckner)
  • Calculates mg of protein and/or ligand required for multiple experiments
  • Gnumeric spreadsheet format (10.4Kb)
  • Excel spreadsheet format (1.4 Mb)
• Microcal ITC Expert (Origin required)
  • Origin project file for iTC200 (323.8 Kb)
  • Origin project file for VP-ITC (141.9 Kb)
  • User manual (464 Kb)
    • Excellent theoretical advice (sample concentration, c-value, ΔCp, buffers)
• Contact Elihu Ihms regarding Origin software access.