Mass Spectrometry Facility

Body

Staff

staff

 

 

 

 

 

Dr. Alicia Friedman | 085 CBEC | 614-292-9646 | friedman.270@osu.edu | u.osu.edu/mass

Jenae Linville | Graduate Teaching Assistant 


FOR MORE INFORMATION ABOUT THE mass spec facility, PLEASE CLICK HERE.

Instruments

Bruker Elute SP HPLC with Bruker Impact II QqTOF Mass Spectrometer

mass

Analysis of small molecules, peptides, proteins, metalloproteins

Mass Spectrometer Specs:

  • Mass Range: 50 - 3000
  • Mass Accuracy < 1 ppm regularly
  • Electrospray Ioniziation (ESI) Source: APCI Source available with advanced notice
  • Collision Cell with N2 flow for Fragmentation (MS/MS or MS2): Multiple modes available to track multiple parent/daughter ions of interest
  • Set up to run Direct Injection and LC/MS modes

HPLC Specs:

  • System includes autosampler, pump, column oven
  • Autosampler compatible 2 mL vials, 96-well plates
  • Pump Flow Rate: 0.01 –5 mL/min, 700 bar pressure max.
  • Autosampler Injection Volumes: 1-45 μL (larger volumes possible with advanced notice)
  • Autosampler Reproducibility: RSD ≤ 0.3% for full loop injections, ≤ 0.5% for partial loop injections
  • Column Oven Range: 10°C above ambient to 90°C with 1°C increments
  • Detection via MS only at this time (possibly adding UV-Vis via DAD in future)

 

Bruker Microflex LRF

maldi

Analysis of biopolymers (peptides, proteins, DNA, RNA, oligosaccharides), organometallics, synthetic polymers

  • Time-of-Flight MS
  • Two Modes of Operation:
    • Reflectron Mode: small molecules, peptides (better resolution)
    • Linear Mode: fragile molecules (oligosaccharides), > 5 kDa
  • Capable of Measuring Negative and Positive Ions
  • 337 nm Nitrogen Laser
  • Mass Range:
    • Reflectron Mode: Up to 20,000 m/z
    • Linear Mode: Up to 500,000 m/z
  • Resolution:
    • Reflectron Mode: > 15,500 for small peptides (i.e. Somatostatin 28 at 3147.5 Da)
    • Linear Mode:
      • > 3,200 for small peptides (i.e. Bombesin at 1619.82 Da)
      • > 1200 for smaller proteins (i.e. Cytochrome C at 12.3 kDa)
      • > 495 for larger proteins (i.e. Protein A at 44.6 kDa)
  • Mass Accuracy:
    • Reflectron Mode:
      • Internal Calibration: < 3 ppm error
      • External Calibration: < 25 ppm error
    • Linear Mode:
      • Internal Calibration: < 45 ppm error
      • External Calibration: < 50 ppm error
  • Limits of Detection:
    • Reflectron Mode: S/N ~ 25 for 1 fmol Glu-Fib (1570.7 Da)
    • Linear Mode: S/N > 130 for 500 fmol BSA (66 kDa)