Bruker Elute SP HPLC with Bruker Impact II QqTOF Mass Spectrometer

Analysis of small molecules, peptides, proteins, metalloproteins

Mass Spectrometer Specs:

• Mass Range: 50 - 3000
• Mass Accuracy < 1 ppm regularly
• Electrospray Ioniziation (ESI) Source: APCI Source available with advanced notice
• Collision Cell with N2 flow for Fragmentation (MS/MS or MS2): Multiple modes available to track multiple parent/daughter ions of interest
• Set up to run Direct Injection and LC/MS modes

HPLC Specs:

• System includes autosampler, pump, column oven
• Autosampler compatible 2 mL vials, 96-well plates
• Pump Flow Rate: 0.01 –5 mL/min, 700 bar pressure max.
• Autosampler Injection Volumes: 1-45 μL (larger volumes possible with advanced notice)
• Autosampler Reproducibility: RSD ≤ 0.3% for full loop injections, ≤ 0.5% for partial loop injections
• Column Oven Range: 10°C above ambient to 90°C with 1°C increments
• Detection via MS only at this time (possibly adding UV-Vis via DAD in future)

Bruker Microflex LRF

Analysis of biopolymers (peptides, proteins, DNA, RNA, oligosaccharides), organometallics, synthetic polymers

• Time-of-Flight MS
• Two Modes of Operation:
  • Reflectron Mode: small molecules, peptides (better resolution)
  • Linear Mode: fragile molecules (oligosaccharides), > 5 kDa
• Capable of Measuring Negative and Positive Ions
• 337 nm Nitrogen Laser
• Mass Range:
  • Reflectron Mode: Up to 20,000 m/z
  • Linear Mode: Up to 500,000 m/z
• Resolution:
  • Reflectron Mode: > 15,500 for small peptides (i.e. Somatostatin 28 at 3147.5 Da)
  • Linear Mode:
    • > 3,200 for small peptides (i.e. Bombesin at 1619.82 Da)
    • > 1200 for smaller proteins (i.e. Cytochrome C at 12.3 kDa)
    • > 495 for larger proteins (i.e. Protein A at 44.6 kDa)
• Mass Accuracy:
  • Reflectron Mode:
    • Internal Calibration: < 3 ppm error
    • External Calibration: < 25 ppm error
  • Linear Mode:
    • Internal Calibration: < 45 ppm error
    • External Calibration: < 50 ppm error
• Limits of Detection:
  • Reflectron Mode: S/N ~ 25 for 1 fmol Glu-Fib (1570.7 Da)
  • Linear Mode: S/N > 130 for 500 fmol BSA (66 kDa)