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Bruker Elute SP HPLC with Bruker Impact II QqTOF Mass Spectrometer
Analysis of small molecules, peptides, proteins, metalloproteins
Mass Spectrometer Specs:
- Mass Range: 50 - 3000
- Mass Accuracy < 1 ppm regularly
- Electrospray Ioniziation (ESI) Source: APCI Source available with advanced notice
- Collision Cell with N2 flow for Fragmentation (MS/MS or MS2): Multiple modes available to track multiple parent/daughter ions of interest
- Set up to run Direct Injection and LC/MS modes
HPLC Specs:
- System includes autosampler, pump, column oven
- Autosampler compatible 2 mL vials, 96-well plates
- Pump Flow Rate: 0.01 –5 mL/min, 700 bar pressure max.
- Autosampler Injection Volumes: 1-45 μL (larger volumes possible with advanced notice)
- Autosampler Reproducibility: RSD ≤ 0.3% for full loop injections, ≤ 0.5% for partial loop injections
- Column Oven Range: 10°C above ambient to 90°C with 1°C increments
- Detection via MS only at this time (possibly adding UV-Vis via DAD in future)
Bruker Microflex LRF
Analysis of biopolymers (peptides, proteins, DNA, RNA, oligosaccharides), organometallics, synthetic polymers
- Time-of-Flight MS
- Two Modes of Operation:
- Reflectron Mode: small molecules, peptides (better resolution)
- Linear Mode: fragile molecules (oligosaccharides), > 5 kDa
- Capable of Measuring Negative and Positive Ions
- 337 nm Nitrogen Laser
- Mass Range:
- Reflectron Mode: Up to 20,000 m/z
- Linear Mode: Up to 500,000 m/z
- Resolution:
- Reflectron Mode: > 15,500 for small peptides (i.e. Somatostatin 28 at 3147.5 Da)
- Linear Mode:
- > 3,200 for small peptides (i.e. Bombesin at 1619.82 Da)
- > 1200 for smaller proteins (i.e. Cytochrome C at 12.3 kDa)
- > 495 for larger proteins (i.e. Protein A at 44.6 kDa)
- Mass Accuracy:
- Reflectron Mode:
- Internal Calibration: < 3 ppm error
- External Calibration: < 25 ppm error
- Linear Mode:
- Internal Calibration: < 45 ppm error
- External Calibration: < 50 ppm error
- Reflectron Mode:
- Limits of Detection:
- Reflectron Mode: S/N ~ 25 for 1 fmol Glu-Fib (1570.7 Da)
- Linear Mode: S/N > 130 for 500 fmol BSA (66 kDa)
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**New Instrumentation added 2020**
Bruker Elute SP HPLC with Bruker Impact II QqTOF Mass Spectrometer
Direct Injection and LC/MS modes - for MS, MS/MS, MS2
Bruker Microflex LRF
Improved Mass range and resolution!
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