Mass Spectrometry Facility (MSF)

Mass Spectrometry Facility (MSF)

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Bruker Elute SP HPLC with Bruker Impact II QqTOF Mass Spectrometer

Analysis of small molecules, peptides, proteins, metalloproteins

Mass Spectrometer Specs:

  • Mass Range: 50 - 3000
  • Mass Accuracy < 1 ppm regularly
  • Electrospray Ioniziation (ESI) Source: APCI Source available with advanced notice
  • Collision Cell with N2 flow for Fragmentation (MS/MS or MS2): Multiple modes available to track multiple parent/daughter ions of interest
  • Set up to run Direct Injection and LC/MS modes

HPLC Specs:

  • System includes autosampler, pump, column oven
  • Autosampler compatible 2 mL vials, 96-well plates
  • Pump Flow Rate: 0.01 –5 mL/min, 700 bar pressure max.
  • Autosampler Injection Volumes: 1-45 μL (larger volumes possible with advanced notice)
  • Autosampler Reproducibility: RSD ≤ 0.3% for full loop injections, ≤ 0.5% for partial loop injections
  • Column Oven Range: 10°C above ambient to 90°C with 1°C increments
  • Detection via MS only at this time (possibly adding UV-Vis via DAD in future)

 

Bruker Microflex LRF

Analysis of biopolymers (peptides, proteins, DNA, RNA, oligosaccharides), organometallics, synthetic polymers

  • Time-of-Flight MS
  • Two Modes of Operation:
    • Reflectron Mode: small molecules, peptides (better resolution)
    • Linear Mode: fragile molecules (oligosaccharides), > 5 kDa
  • Capable of Measuring Negative and Positive Ions
  • 337 nm Nitrogen Laser
  • Mass Range:
    • Reflectron Mode: Up to 20,000 m/z
    • Linear Mode: Up to 500,000 m/z
  • Resolution:
    • Reflectron Mode: > 15,500 for small peptides (i.e. Somatostatin 28 at 3147.5 Da)
    • Linear Mode:
      • > 3,200 for small peptides (i.e. Bombesin at 1619.82 Da)
      • > 1200 for smaller proteins (i.e. Cytochrome C at 12.3 kDa)
      • > 495 for larger proteins (i.e. Protein A at 44.6 kDa)
  • Mass Accuracy:
    • Reflectron Mode:
      • Internal Calibration: < 3 ppm error
      • External Calibration: < 25 ppm error
    • Linear Mode:
      • Internal Calibration: < 45 ppm error
      • External Calibration: < 50 ppm error
  • Limits of Detection:
    • Reflectron Mode: S/N ~ 25 for 1 fmol Glu-Fib (1570.7 Da)
    • Linear Mode: S/N > 130 for 500 fmol BSA (66 kDa)
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**New Instrumentation added 2020**

Bruker Elute SP HPLC with Bruker Impact II QqTOF Mass Spectrometer

Direct Injection and LC/MS modes - for MS, MS/MS, MS2

Bruker Microflex LRF

Improved Mass range and resolution!

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Staff

staff

Dr. Alicia Friedman 

BICF Lab: MP 0043
Office: CBEC 085 
614-292-9646
friedman.270@osu.edu
u.osu.edu/mass