Magnetic Resonance Laboratory - NMR and EPR

Magnetic Resonance Laboratory - NMR and EPR

nmr

The NMR Facility is located in the basement of the CBEC Building (092) and Evans Laboratory (EL 0031).  Current instrumentation includes six solution-state spectrometers and one solid-state spectrometer. Each instrument is configured with unique capabilities and optimized for a large variety of experiments to meet the needs of interested research groups, undergraduate students, and industrial clients.

The EPR Facility includes a Bruker EMX-Plus continuous-wave (CW) EPR spectrometer, a Bruker E580 pulsed EPR spectometer, and a benchtop Bruker ESR5000 CW EPR spectrometer. The EMX and E580 systems are both equipped with cryogen free He crysotats capable of measuring samples between 6 - 300 K with optical window access for measurement of irradiated samples and operate at X-band frequencies (~9.6 GHz) . The ESR5000 is a X-band CW instrument routinely used for measurement of sample temperatures between 77 - 300 K.

 

Routine maintenance and repairs are provided by the NMR Facility Staff and the CBC Instrumentation Support Group.

 

Higher field instruments optimized for bioNMR applications are part of the Campus Chemical Instrument Center (CCIC) and the National Gateway Ultrahigh Field NMR Center, managed and operated by the Office of Research.

Chen, D., Zhu, X., Ilavsky, J., Whitmer, T.L., Hatzakis, E., Jones, O., Campanella, O.; Polyphenols weaken pea protein gel by formation of large aggregates with diminished non-covalent interactions, Biomacromolecules 2021, 22, 1001. DOI: 10.1021/acs.biomac.0c01753.

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Chase M. Kayrouz, Yeying Zhang, Tiffany M. Pham, and Kou-San Ju; Genome Mining Reveals the Phosphonoalamide Natural Products and a New Route in Phosphonic Acid Biosynthesis; ACS Chemical Biology 2020 15 (7), 1921-1929 DOI: 10.1021/acschembio.0c00256.

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R. Z. Pavlović, L. Zhiquan, M. Güney, R. F. Lalisse, R. G. Hopf, J. Gallucci, C. Moore, H. Xie, C. M. Hadad, J. D. Badjić; Multivalent C−H⋅⋅⋅Cl/Br−C Interactions Directing the Resolution of Dynamic and Twisted Capsules; Chem. Eur. J. 201925, 13124. DOI: 10.1002/chem.201903006.

NMR figure
NMR figure

Spectrometers Available

The NMR Facility is located in the basement of the CBEC Building (092) and Evans Laboratory (EL 0031).  Current instrumentation includes six solution-state spectrometers and one solid-state spectrometer. Each instrument is configured with unique capabilities and optimized for a large variety of experiments to meet the needs of interested research groups, undergraduate students, and industrial clients.

Solution NMR

600 MHz Avance III HD -  This is our most versatile instrument in the facility, capable of a large number of NMR experiments ranging from routine to advance multidimensional, multinuclear NMR, water suppression, and variable temperature.  Most experiments can be acquired using automation.    

400 MHz Avance NEO  - This instrument is a walkup instrument utilizing a SampleJet and a liquid nitrogen cooled probe (Prodigy) enhanced sensitivity and for high sample through-put.

400 MHz Avance III  - This is a walk-up instrument which utilizes the SampleJet for high sample through-put.

400 MHz DPX  - Currently, we are using this instrument for routine 1H, 13C, and 31P experiments and variable temperature studies.

250 MHz DPX  - Primarily a walk-up instrument equipped with a quarternary probe for detection of X (carbon, phosphorus, and boron) nuclei.

80 MHz Benchtop - Primarily for demonstration & education.

 

Solid-state NMR

500 MHz Varian in CBEC 92 - This instrument is available via collaboration with the NMR Laboratory staff. 

 

Electron Paramagnetic Resonance - EPR

The EPR Facility includes a Bruker EMX-Plus continuous-wave (CW) EPR spectrometer, a Bruker E580 pulsed EPR spectrometer, and a benchtop Bruker ESR5000 CW EPR spectrometer. The EMX and E580 systems are both equipped with cryogen free crysotats capable of measuring samples between 6 - 300 K with optical window access for measurement irradiated samples and operate at X-band frequencies (~9.6 GHz) . The ESR5000 is a X-band CW instrument routinely used for measurement of samples at temperatures between 77 - 300 K.

EMX-Plus EPR – CBEC 081

ESR5000 Benchtop EPR – CBEC 081

E580 Pulsed EPR - Evans Laboratory 031

Staff

staff2

Dr. Dan Conroy

Magnetic Resonance Laboratory Manager - NMR

conroy.120@osu.edu

u.osu.edu/cbcnmr


Luke Lewis

Dr. Luke Lewis

Magnetic Resonance Laboratory Manager - EPR

lewis.2667@osu.edu

u.osu.edu/cbcnmr